gdf9  (R&D Systems)

Journal: Cell communication and signaling : CCS

Article Title: The dose-dependent dual effects of alpha-ketoglutarate (AKG) on cumulus oocyte complexes during in vitro maturation.

doi: 10.1186/s12964-024-01827-z

Figure Lengend Snippet: Fig. 5 Effect of AKG on cell-cell communication between cumulus and oocyte (A) The interaction weight plot of COCs. The thickness of the line indicates the number of interactions, and the stronger the interaction weight/intensity between the two cell types. Bar plot showed the total interaction weight of COCs in each group. (B) The up-regulated (increased) signaling ligand-receptor pairs were identified by comparing the communication probabilities in AKG30 samples and other samples. (C) Immunoblotting of GDF9 in control, 30 and 750 µM AKG treated oocytes. (D) The expression of GDF9 in oocytes, expressed using log2(TPM + 1), n = 8. (E) the fluorescence intensity of oocytes Calcein-AM to indicate the changes of Cumulus cell-oocyte gap junction permeability during oocyte maturation, n = 9. (F) Representative images of the fluorescence intensity of F-actin on the zona pellucida to indicate the changes of transzonal projections (TZPs) during oocyte maturation. (G) Line graph showed the changes of TZPs during oocyte maturation

Article Snippet: In some experiments, the culture medium was supplemented with 100 ng/mL GDF9 protein (MCE, Shanghai, China) or 1 μM carbenoxolone disodium (CBX) (MCE, Shanghai, China).

Techniques: Western Blot, Control, Expressing, Fluorescence, Permeability

Journal: Cell communication and signaling : CCS

Article Title: The dose-dependent dual effects of alpha-ketoglutarate (AKG) on cumulus oocyte complexes during in vitro maturation.

doi: 10.1186/s12964-024-01827-z

Figure Lengend Snippet: Fig. 6 Effects of AKG and GDF9 on cumulus cells expansion. (A) The expression of cumulus expansion related genes HAS2, PTX3, CD44 and HMMR in cumulus cells expressed by log2 (TPM + 1), n = 3. (B) Immunoblotting of HAS2 in control, 30 and 750 µM AKG treated cumulus cells. (C) Representative images of the COCs after 22 h of culture, Scale bar = 200 µM, n = 30. (D) Relative cumulus expansion area n = 30. (E) Cumulus expansion index, n = 30. (F) qPCR results showed the expression of cumulus expansion related genes HAS2, PTX3, CD44 and HMMR in cumulus, n = 3

Article Snippet: In some experiments, the culture medium was supplemented with 100 ng/mL GDF9 protein (MCE, Shanghai, China) or 1 μM carbenoxolone disodium (CBX) (MCE, Shanghai, China).

Techniques: Expressing, Western Blot, Control

Journal: Heliyon

Article Title: Comparing GDF9 in mature follicles and clinical outcomes across different PCOS phenotype

doi: 10.1016/j.heliyon.2024.e29879

Figure Lengend Snippet: Distribution of GDF9 in FF from the three PCOS phenotypes and control group. Data are presented as mean ± SD. d P < 0.01, compared with Control Group; a P < 0.01, compared with Control Group; b P < 0.05, compared with PCOS A; c P < 0.05, compared with PCOS B.

Article Snippet: The FF was diluted 1:5 in phosphase-buffered saline (PBS) and measured using a human GDF9 ELISA Kit (Elabscience, Wuhan, China) according to the manufacture's instructions.

Techniques: Control

Journal: Heliyon

Article Title: Comparing GDF9 in mature follicles and clinical outcomes across different PCOS phenotype

doi: 10.1016/j.heliyon.2024.e29879

Figure Lengend Snippet: Expression of GDF9 in the CCs. (a) Immunohistochemical staining of GDF9 expressed in the control group, phenotype A, phenotype B and phenotype D. (Original magnification: × 400, bars, 50 μm). (b) Mean density of GDF9 in CCs of the three PCOS phenotypes and control group, a P < 0.01, compared with Control Group; b P < 0.01, compared with PCOS D; c P < 0.01, compared with Control Group; d P < 0.01, compared with PCOS D.

Article Snippet: The FF was diluted 1:5 in phosphase-buffered saline (PBS) and measured using a human GDF9 ELISA Kit (Elabscience, Wuhan, China) according to the manufacture's instructions.

Techniques: Expressing, Immunohistochemical staining, Staining, Control

Journal: Heliyon

Article Title: Comparing GDF9 in mature follicles and clinical outcomes across different PCOS phenotype

doi: 10.1016/j.heliyon.2024.e29879

Figure Lengend Snippet: Analysis of multiple factors affecting blastocyst formation and clinical pregnancy rate in the PCOS phenotypes.

Article Snippet: The FF was diluted 1:5 in phosphase-buffered saline (PBS) and measured using a human GDF9 ELISA Kit (Elabscience, Wuhan, China) according to the manufacture's instructions.

Techniques: Control

Journal: Cell communication and signaling : CCS

Article Title: GDF9 His209GlnfsTer6/S428T and GDF9 Q321X/S428T bi-allelic variants caused female subfertility with defective follicle enlargement.

doi: 10.1186/s12964-024-01616-8

Figure Lengend Snippet: Fig. 5 Effects of Gdf9 variants on antral follicle development after PMSG stimulation. A Appearance of Gdf9wt/wt, Gdf9S415T/S415T and Gdf9Q308X/

Article Snippet: To confirm the regulatory role of GDF9 on P4HA2, hGCs were incubated with 200ng/ml recombinant mature GDF9 (HY-P72425, MCE, USA) with or without 10μM SB431542 ( HY-10431, MCE, USA), an inhibitor of GDF9 receptor ALK5, for 24-48 hour and then RNA and protein were extracted, respectively.

Techniques: